What this is for: Virus dosing by multiplicity of infection — forward: µL of stock for a target MOI; reverse: the MOI a given volume actually delivered.
The formula: MOI = infectious units added ÷ number of cells; volume (mL) = MOI × cells ÷ titer (PFU/mL)
How to use:
- For a planned infection, use section A: enter the number of cells, virus titer in PFU/mL, and desired MOI.
- Read the total PFU needed and the stock volume to add in mL and µL.
- To check an infection already done, use section B: enter the volume added, titer, and number of cells.
- Read the PFU added and the actual MOI.
A. Forward — cells + titer + MOI → volume to add
MOI = infectious units (PFU) added / number of cells.
Total PFU needed
Volume of stock (mL)
Volume of stock (µL)
B. Reverse — volume added + titer + cells → actual MOI
µL
PFU added
ACTUAL MOI
Prefer to work offline or keep a copy? The download link is right below.
Download the MOI Calculator workbook (.xlsx)
Download the MOI Calculator workbook (.xlsx)
Notes & limits (from the workbook): MOI = infectious units (PFU) added / number of cells. Section A (forward): how much virus stock to add. Section B (reverse): the MOI you actually got.