What this is for: From cells-per-well, well count and your counted stock concentration, gives the stock and medium volumes for a seeding job, with overage.
The formula: stock volume (mL) = (cells/well × wells × (1 + overage)) ÷ stock concentration (cells/mL)
How to use:
- Choose the plate format; the volume per well fills in from the format.
- Enter the desired cells per well, the number of wells to seed, and your counted stock concentration in cells/mL.
- Set the overage percentage.
- Read the total cells needed, stock suspension to use, total seeding volume, medium to add, and stock per well.
Cell Seeding Calculator
Pick a plate format (typical volumes fill in), enter cells per well, number of wells and your counted stock concentration — get stock + media volumes, with overage.
%
Volume per well (mL)
Total cells needed (with overage)
Stock suspension to use (mL)
Total seeding volume (mL)
Media to add (mL)
Stock per well (µL)
| Plate format | Wells per plate | Volume per well (mL) | Growth area (cm²) |
|---|---|---|---|
| 6-well | 6 | 2 | 9.6 |
| 12-well | 12 | 1 | 3.5 |
| 24-well | 24 | 0.5 | 1.9 |
| 48-well | 48 | 0.25 | 0.95 |
| 96-well | 96 | 0.1 | 0.32 |
Prefer to work offline or keep a copy? The download link is right below.
Download the Cell Seeding Calculator workbook (.xlsx)
Download the Cell Seeding Calculator workbook (.xlsx)
Notes & limits (from the workbook): Pick a plate format (typical volumes fill in), enter cells per well, number of wells and your counted stock concentration — get stock + media volumes, with overage. Edit the reference table in the workbook if your lab uses different per-well volumes.