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Buffer & Solution Maker

Up to 8 components with automatic molecular-weight lookup and one shared volume. PBS, TAE 50X and TBS presets included. The same math as the Excel workbook, live in your browser.

What this is for: Builds a multi-component buffer or medium for one shared final volume, with each component's molecular weight looked up automatically.

The formula: grams per component = molarity (mol/L) × volume (L) × MW (g/mol)

How to use:

  1. Enter one shared final volume and choose L or mL.
  2. For each of up to 8 components, pick the chemical — its MW fills in automatically.
  3. Enter that component's target molarity (mol/L).
  4. Read the grams to weigh shown for each component.
  5. For PBS 1X, TAE 50X or TBS 1X, copy the preset molarities listed below the calculator into the table.

Buffer Maker — up to 8 components, one shared volume

Enter ONE shared final volume, then up to 8 components: pick each chemical (MW fills in automatically), enter its target molarity, and read off the grams to weigh.

Volume (L):
MW:
Grams to weigh (g): — PBS 1X
MW:
Grams to weigh (g): — PBS 1X
MW:
Grams to weigh (g): — PBS 1X
MW:
Grams to weigh (g): — PBS 1X
MW:
Grams to weigh (g):
MW:
Grams to weigh (g):
MW:
Grams to weigh (g):
MW:
Grams to weigh (g):

Preset Recipes — standard molarities (copy into the table above)

Grams per litre are computed from the same chemical database (molarity × MW × 1 L).

PBS 1X (per 1 L, pH 7.4) — Sodium chloride, 0.137 M
PBS 1X — Potassium chloride, 0.0027 M
PBS 1X — Sodium phosphate dibasic (anhydrous), 0.01 M
PBS 1X — Potassium phosphate monobasic, 0.0018 M
TAE 50X stock (per 1 L of 50X; use at 1X) — Tris base, 2 M
TAE 50X — Acetic acid (glacial), 1 M
TAE 50X — EDTA disodium salt dihydrate, 0.05 M
TBS 1X (per 1 L, pH 7.6) — Tris base, 0.02 M
TBS 1X — Sodium chloride, 0.15 M
Prefer to work offline or keep a copy? The download link is right below.
Download the Buffer & Solution Maker workbook (.xlsx)
Notes & limits (from the workbook): Enter ONE shared final volume, then up to 8 components: pick each chemical (MW fills in automatically), enter its target molarity, and read off the grams to weigh. Preset recipes (PBS, TAE 50X, TBS) are listed below the calculator in the workbook — copy their molarities into the table. TAE note: acetic acid is a liquid — 1.0 M ≈ 57.1 mL glacial acetic acid per litre; EDTA is normally added as 100 mL of 0.5 M EDTA pH 8.0 per litre of 50X.